Title: Circulating Mirnas Reflect Early Myocardial Injury and Recovery after Heart Transplantation
نویسندگان
چکیده
1. Besides miR-133a, miR-133b and miR-208a, there are several other muscle-specific miRNAs, such as miR-1, etc. Why only these miRNAs were used in this study? 2. P2: qRT-PCR is not quantitative real-time polymerase chain reaction, but quantitative reverse transcription-polymerase chain reaction. 3. P6: “To date, no housekeeping miRNAs have been established and validated to normalize for the miRNAs content.” It’s wrong. Actually, miR-16 has been used as a reference in the detection of body fluid miRNAs (Cui L, et al. Cancer. 2013 May 1;119(9):1618-26). Should gave some discussion about this.
منابع مشابه
Circulating miRNAs reflect early myocardial injury and recovery after heart transplantation
BACKGROUND MicroRNAs (miRNAs) are short, single-stranded and non-coding RNAs, freely circulating in human plasma and correlating with vary pathologies. In this study, we monitored early myocardial injury and recovery after heart transplantation by detecting levels of circulating muscle-specific miR-133a, miR-133b and miR-208a. METHODS 7 consecutive patients underwent heart transplantation in ...
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